This Automated Library Preparation System (ALPS) was designed as a stand-alone tabletop unit that includes all standard library prep components to enable library prep for molecular diagnostics in a space-efficient format. This small package library preparation system delivers a fully automated assay workflow, providing process efficiencies and improved systematic liquid handling accuracy. The user places consumables (which Simplexity has also designed) in the system and the system delivers a processed sample, ready for sequencing. Harnessing our expertise in printer drive train assemblies, this unit delivers a viable lower cost solution to an expanding market sector.
THE PRODUCT
- A sophisticated breadboard incorporating all steps of a standard Library Prep (LP) workflow for Next Generation Sequencing (NGS)
- Used to perform guard band studies and de-risk automation of the Library Prep Assay Protocol before green lighting final product development
- Includes integrated stations for standard 96 wellplate
- Thermocycler
- Non-contact Dispense array
- Reagent management system
- Aspiration/Waste management station
- Bead removal station
- Embedded motion system to move between all stations
- Dedicated, on demand pressure and vacuum supply system
TOOL DEVELOPMENT
- Worked closely with the client design team to integrate single-station breadboard designs from the client with Simplexity’s original designs and combine them into a single, integrated fully automated system. This included mechanical, electrical, firmware, and software engineering.
- Developed production level consumables for testing in the system along with fixtures and protocols for their assembly.
- Generated application programming interface (API) software for both independent control and integration with other lab automation tools.
Software: Design Complete
Hardware: Pre-production units for design verification
MFG. Readiness: CM schedule and budget, Unit build tracking